Histological evaluation of sciatic nerve anastomosis wrapped by freeze dried human amniotic membrane in rat sciatic nerve defect model

Published by Admin Journal on

No. 2



In peripheral nerve injury, poor environment
condition, such as excessive inflammation and scar formation
surrounding an injured nerve, will prevent nerve regeneration
process. Human amniotic membrane with low imunogenicity
has proven to decrease fibrous formation in myocardial
infarction and liver fibrosis in animal models. Recent studies
showed that the human amniotic membrane can suppresses the
expression of the potent pro-inflammatory cytokines.

Materials and methods

This research was conducted on thirty Sprague
Dawley rats and divided into 2 groups. In control group, the
sciatic nerve was totally transected and repaired by epineural
microsutures. In the experimental group, after the anastomosis
of injured nerve, the nerve was wrapped using freeze dried
human amniotic membrane. Subsequently, it was evaluated
using Hematoxylin Eosin and Mason’s Trichrome staining on
day 7th, 14th and 21st after anastomosis and then converted into
inflammation and fibrosis score.


Freeze dried human amniotic membrane decreases
the inflammation score of the injured nerve only in the first
week after anastomosis procedure (p<0,05). However, there
was no significant difference of fibrosis score during 3 weeks
after application.


Freeze dried human amniotic membrane decreases inflammation reaction within the first week after anastomosis in Sciatic nerve defect model.

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