The Effect of Vitamin C, E, and B12 on the reduction of Ischemic Skeletal Muscle Damage on Rat (Rattus norvegicus) due to Reperfusion Injury

Effect of Vitamins on Muscles Due to Ischemia-Reperfusion Injury

Authors

  • Savero Iman Hari Suko Department of Orthopaedics, Prof Dr. R. Soeharso Orthopaedic Hospital Surakarta
  • Pamudji Utomo Department of Orthopaedic and Traumatology, Universitas Sebelas Maret, Prof. Dr. R. Soeharso Orthopaedic Hospital, Surakarta, Indonesia
  • Mujadid Idulhaq Department of Orthopaedic and Traumatology, Universitas Sebelas Maret, Prof. Dr. R. Soeharso Orthopaedic Hospital, Surakarta, Indonesia
  • Cakradenta Yudha Poetera Department of Orthopaedic and Traumatology, Universitas Sebelas Maret, Prof. Dr. R. Soeharso Orthopaedic Hospital, Surakarta, Indonesia

DOI:

https://doi.org/10.31282/joti.v4n2.76

Keywords:

antioxidants, myocyte damage, reperfusion injury

Abstract

Introduction: Tourniquet is usually used in limb surgery, both orthopaedic surgery and plastic surgery.1 The use of pneumatic tourniquets has become a standard for creating bloodless fields in the upper and the lower limb surgery. However, the use of tourniquets is dangerous because it causes complications. Complications of tourniquet use occur due to mechanical suppression of the underlying tissue, ischemia, and reperfusion effects. This causes local and systemic complications.

Objectives: This study aims to determine the effect of vitamin C, vitamin E and B12 in reducing rat skeletal muscle damage due to tourniquets.

Methods: This research is an experimental laboratory with a post-test-controlled group design. The sample consisted of 20 female Wistar rats divided into 4 groups, where each group consisted of controlled group, vitamin C group, vitamin E group, and vitamin B12 group. All the rats were anesthetized and then a tourniquet (orthodontic rubber) was placed on the thigh of each rat for 2 hours and continued reperfusion was performed for 1 hour after the tourniquet was released. Histological picture of the muscle cells (myocytes) was observed and the number of the damaged cells and the healthy ones were counted. Data were analyzed using the One-Way ANOVA test (α = 0.05) and continued with the Post Hoc Multiple Comparisons (LSD) test (α = 0.05).

Results: The result of One-Way ANOVA test showed significant differences between the four treatment groups. The result of Post Hoc test showed significant differences between the control group and the groups P2, P3 and P4. Whereas the P3 group showed significant difference with the P2 and P4 groups.

Conclusions: Oral administration of vitamins C, E and B12 for 5 days reduces histological damage of rat myocytes in ischemic reperfusion injuries due to tourniquet. Vitamin E has the best effectiveness compared to vitamins C and B12.

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References

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Additional Files

Published

2021-08-31

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Section

Original Research Article