The effect of amnion membrane on the function and histopathology of the sciatic nerve in nerve crush injury in sprague dawley rat
DOI:
https://doi.org/10.31282/joti.v2n1.36Keywords:
Peripheral nerve injury, Amniotic membrane, Nerve Crush InjuryAbstract
Introduction:
The aim of this study is to evaluate the validity of the Amniotic Membrane for nerve regeneration in rat sciaticvnerve crush injury, both functionally and histologically.
Methods:
In this study, data was obtained from 18 healthy Sprague Dawley rats. The intervention given was a unilateral right side sciatic nerve crush injury by pinching with a forcepand, and then the rats were observed with different methods: Group I (control group): 9 rats with damaged nerve left untreated with amniotic membrane. Group II: 9 rats, with damaged nerve treated with amnion membrane. The evaluation was performed over 21 days post-operatively for both functionally and histologically.
Results:
There were significant difference in functional parameters evaluated by walking track analysis between Group I and Group II at day 5, 7, 14, and 21 (P<0.05). Histological features of Group II, after 21 days, showed that there was complete nerve regeneration and reconnection. Histological results showed that there was significant difference between Group I and Group II for axonal degeneration (P=0.0024), nerve regeneration (P=0.016), and lower inflammation reaction (P=0.016). However, there was no significant difference in nerve diameter (P=0.23).
Conclusion:
It can be concluded from the results of this study that the application of amniotic membrane in crush injury nerve damage gave better outcome, both functionally and histologically, compared to the damaged nerve that was left untreated with amniotic membrane and this could be used as an alternative technique to improve nerve healing in nerve crush injury
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