Histological evaluation of sciatic nerve anastomosis wrapped by freeze dried human amniotic membrane in rat sciatic nerve defect model

Authors

  • Hastomo Agung Wibowo Departement Orthopaedic&Traumatology, Faculty of Medicine, Universitas Sebelas Maret Prof.Dr.dr.R.Soeharso Orthopaedic Hospital, Solo, Indonesia
  • Pamudji Utomo Departement Orthopaedic&Traumatology, Faculty of Medicine, Universitas Sebelas Maret Prof.Dr.dr.R.Soeharso Orthopaedic Hospital, Solo, Indonesia
  • Mujadid Idulhaq Departement Orthopaedic&Traumatology, Faculty of Medicine, Universitas Sebelas Maret Prof.Dr.dr.R.Soeharso Orthopaedic Hospital, Solo, Indonesia

Keywords:

peripheral nerve injury, freeze dried human amniotic membrane, inflammation score, fibrosis score

Abstract

Introduction:

In peripheral nerve injury, poor environment condition, such as excessive inflammation and scar formation surrounding an injured nerve, will prevent nerve regeneration process. Human amniotic membrane with low imunogenicity has proven to decrease fibrous formation in myocardial infarction and liver fibrosis in animal models. Recent studies showed that the human amniotic membrane can suppresses the expression of the potent pro-inflammatory cytokines.

Methods:

This research was conducted on thirty Sprague Dawley rats and divided into 2 groups. In control group, the sciatic nerve was totally transected and repaired by epineural microsutures. In the experimental group, after the anastomosis of injured nerve, the nerve was wrapped using freeze dried human amniotic membrane. Subsequently, it was evaluated using Hematoxylin Eosin and Mason’s Trichrome staining on day 7th, 14th and 21st after anastomosis and then converted into inflammation and fibrosis score.

Results:

Freeze dried human amniotic membrane decreases the inflammation score of the injured nerve only in the first week after anastomosis procedure (p<0,05). However, there was no significant difference of fibrosis score during 3 weeks after application.

Conclusion:

Freeze dried human amniotic membrane decreases inflammation reaction within the first week after anastomosis in Sciatic nerve defect model.

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References

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Additional Files

Published

2018-08-20